What is the best way to dissolve peptides?
The solubility of a given peptide varies depending on its amino acid sequence and modifications. LexinPharma
purifies peptides by RP-HPLC using a water and acetonitrile gradient. Here are some general tips for dissolving
peptides:
Sonication increases solubility. 10% acetic acid in the solvent will help dissolve basic peptides (Isoelectric Point, PI >7). 10% ammonium bicarbonate will help dissolve acidic peptides. (PI <7)
For very hydrophobic peptides which are sparingly soluble in aqueous solutions, water-miscible organic solvents
(such as dimethyl sulfoxide (DMSO), isopropanol, methanol, and acetonitrile) should be used first. Once the
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