What peptide purification methods do you use?
Theoretical net peptide content (calculated assuming that counterions are the only non-peptide components present in your In most cases, we use preparative RP-HPLC for peptide purification. Occasionally, ion exchange chromatography (IEX) may be utilized as well. IEX is particularly useful in the case of pegylated peptides for removal of the free, unreacted PEG. Additionally, size exclusion (SEC) may be used to remove high-molecular-weight impurities and polymers such as with multi-disulfide peptides.
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